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An increase in sperm motility is seen in vitro when acetyl-L-carnitine is added to test the samples

Further studies are warranted to elucidate the potential regulatory effects of inflammatory mediators on lactylation

The luciferase activity measurements were performed by transferring 72 L of assay buffer (25 mM glycylglycine, 5 mM KPO 4 , 50 mM MgSO 4 , 10 mM EGTA, 2% ATP, 1 mM dithiotreitol) to a white 96-well plate and adding 20 L of the cell-lysis suspension with subsequent adding of 40 L D-Luciferin and immediate measuring using the Berthold LB960 Centro XS3 plate luminometer

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